RBS

Part:BBa_K390001:Design

Designed by: Cody Tramp   Group: iGEM10_Utah_State   (2010-08-01)


5'UTR and native RBS from psbA2 in Synechocystis sp. PCC 6803


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to go with the full region from the promoter to the start codon. This means that, with the addition of the biobrick scar, the RBS may be located farther from the start codon then is optimal. We plan to determine optimum spacing of the RBS in Synechocystis sp. PCC 6803 in future work.


Source

Genomic sequences from Synechocystis sp. PCC 6803

References